产品名称
山羊抗兔IgG H&L (HRP)
参阅全部 IgG 二抗
宿主
Goat
靶标种属
Rabbit
经测试应用
适用于: IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, ICC, IHC-Frmore details
免疫原
Rabbit IgG, whole molecule
偶联物
HRP
形式
Liquid
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Please see notes section. Store In the Dark.
存储溶液
pH: 7.20
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
常规说明
HRP conjugated anti-rabbit secondary antibody optimized for western blot and immunohistochemistry. Some customers reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Our Abpromise guarantee covers the use of ab6721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/1000. | |
| WB | 1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283). Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances. | |
| ELISA | 1/120000. | |
| Immunomicroscopy | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. | |
| ICC | 1/1000 - 1/5000. | |
| IHC-Fr | 1/1000. |