Thermo Scientific Spectra Multicolor High Range Protein Ladder is a mixture of eight blue-, green-, and orange-stained proteins (40 to 300 kDa) for use as size standards for high-MW proteins in gel electrophoresis and western blotting. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
Applications
• Monitoring of protein migration during SDS-PAGE
• Verifying western transfer efficiency
• Approximate sizing of proteins on SDS-polyacrylamide gels and western blots
• Locating a protein of interest for excision from an unstained preparative gel
规格
| Detection Method: | colorimetric, NIR fluorescence (700nm channel), RGB fluorescence (555nm channel) |
|---|---|
| Number of Markers: | 8 |
| Product Line: | Spectra™ |
| Recommended Applications: | Tris-acetate gels |
| Size Range: | 40 to 300 kDa |
| System Type: | SDS-PAGE |
| 分子量: | 300, 250, 180, 130, 100, 70, 50, 40 kDa |
| 染色剂类型: | 3 colors: Blue, Orange, Green |
| 数量.: | 2 x 250 mL |
内容及储存
Contents: two vials of 250 µL each
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C