β-Actin Antibody (C4) is a high quality monoclonal beta-Actin antibody suitable for the detection of the beta-Actin protein of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin. β-Actin Antibody (C4) is available as both the non-conjugated anti-beta-Actin antibody form, as well as multiple conjugated forms of anti-beta-Actin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. β-Actin belongs to the actin protein family, which is expressed by all eukaryotic cells. β-Actin is highly conserved among species, making the β-Actin Antibody (C4) suitable for detection of β-Actin expressed in both mammalian and non-mammalian cells. The actin protein often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Due to the cellular localization of β-Actin protein, the β-Actin Antibody (C4) shows cytoplasmic and membrane staining. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
抗-beta-Actin 抗体 C4 是小鼠单克隆 IgG1 κ，β-Actin抗体, 在10,720篇文献中引用，规格为200 µg/ml
raised against gizzard Actin of chicken origin
抗-beta-Actin 抗体 (C4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum 和Physarum polycephalum 来源的 β-Actin; 可能与 all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)产生交联反应
抗-beta-Actin 抗体 (C4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
可用于凝胶迁移和ChIP的TransCruz试剂(sc-47778 X, 200 µg/0.1 ml)