William's E 培养基最初由 Williams 和 Gunn 开发作为减血清补充培养基，用于成年大鼠肝脏上皮细胞的长期细胞培养。Gibco™ William's E 培养基也可用于培养不同物种的原代肝细胞以及人源 HepaRG 细胞。
William's E 培养基的改良方式如下：
| 不含 |
| •酚红 |
| •L-谷氨酰胺 |
| • HEPES |
Gibco William's E 培养基是早期培养基（富含氨基酸，葡萄糖含量加倍）的改良产品。William's E 培养基含有独特的成分，包括锌、铁、锰、非必需氨基酸、还原剂谷胱甘肽和脂质亚油酸甲酯
两地 cGMP 生产
为确保供应链的稳定，在位于纽约格兰德岛和英国苏格兰的两家独立工厂生产 Gibco William's E 培养基。两家工厂均符合 cGMP 生产要求，并通过 ISO 13485 认证，是在 FDA 登记的医疗器械生产商。
William's E 培养基不含蛋白或生长因子。因此，William's E 培养基需要补充剂，通常添加 5%-10% 胎牛血清 (FBS)。William's E 培养基使用碳酸氢钠缓冲系统 (2.2 g/L)，因此需要 5%-10% CO2 环境来维持生理 pH 值。
A12176 - Williams' Medium E, no Phenol Red
Catalog Number(s)
| Components | Molecular Weight | Concentration (mg/L) | mM |
|---|---|---|---|
| Amino Acids | |||
| Glycine | 75.0 | 50.0 | 0.6666667 |
| L-Alanine | 89.0 | 90.0 | 1.011236 |
| L-Arginine | 174.0 | 50.0 | 0.28735632 |
| L-Asparagine-H2O | 150.0 | 20.0 | 0.13333334 |
| L-Aspartic acid | 133.0 | 30.0 | 0.22556391 |
| L-Cysteine | 121.0 | 40.0 | 0.3305785 |
| L-Cystine 2HCl | 313.0 | 26.07 | 0.08329073 |
| L-Glutamic Acid | 147.0 | 50.0 | 0.34013605 |
| L-Histidine | 155.0 | 15.0 | 0.09677419 |
| L-Isoleucine | 131.0 | 50.0 | 0.3816794 |
| L-Leucine | 131.0 | 75.0 | 0.57251906 |
| L-Lysine hydrochloride | 183.0 | 87.46 | 0.47792348 |
| L-Methionine | 149.0 | 15.0 | 0.10067114 |
| L-Phenylalanine | 165.0 | 25.0 | 0.15151516 |
| L-Proline | 115.0 | 30.0 | 0.26086956 |
| L-Serine | 105.0 | 10.0 | 0.0952381 |
| L-Threonine | 119.0 | 40.0 | 0.33613446 |
| L-Tryptophan | 204.0 | 10.0 | 0.04901961 |
| L-Tyrosine disodium salt dihydrate | 261.0 | 50.65 | 0.19406131 |
| L-Valine | 117.0 | 50.0 | 0.42735043 |
| Vitamins | |||
| Ascorbic Acid | 176.0 | 2.0 | 0.011363637 |
| Biotin | 244.0 | 0.5 | 0.0020491802 |
| Choline chloride | 140.0 | 1.5 | 0.010714286 |
| D-Calcium pantothenate | 477.0 | 1.0 | 0.002096436 |
| Ergocalciferol | 397.0 | 0.1 | 2.5188917E-4 |
| Folic Acid | 441.0 | 1.0 | 0.0022675737 |
| Menadione sodium bisulfate | 276.0 | 0.01 | 3.6231882E-5 |
| Niacinamide | 122.0 | 1.0 | 0.008196721 |
| Pyridoxal hydrochloride | 204.0 | 1.0 | 0.004901961 |
| Riboflavin | 376.0 | 0.1 | 2.6595744E-4 |
| Thiamine hydrochloride | 337.0 | 1.0 | 0.002967359 |
| Vitamin A (acetate) | 328.0 | 0.1 | 3.0487805E-4 |
| Vitamin B12 | 1355.0 | 0.2 | 1.4760147E-4 |
| alpha Tocopherol phos. Na salt | 554.7 | 0.01 | 1.8027762E-5 |
| i-Inositol | 180.0 | 2.0 | 0.011111111 |
| Inorganic Salts | |||
| Calcium Chloride (CaCl2) (anhyd.) | 111.0 | 200.0 | 1.8018018 |
| Cupric sulfate (CuSO4-5H2O) | 250.0 | 1.0E-4 | 3.9999998E-7 |
| Ferric sulfate (FeSO4-7H2O) | 278.0 | 1.0E-4 | 3.5971223E-7 |
| Magnesium Sulfate (MgSO4) (anhyd.) | 120.0 | 97.67 | 0.8139166 |
| Manganese Sulfate (MnSO4-H20) | 169.0 | 1.0E-4 | 5.9171595E-7 |
| Potassium Chloride (KCl) | 75.0 | 400.0 | 5.3333335 |
| Sodium Bicarbonate (NaHCO3) | 84.0 | 2200.0 | 26.190475 |
| Sodium Chloride (NaCl) | 58.0 | 6800.0 | 117.24138 |
| Sodium Phosphate monobasic (NaH2PO4) anhydrous | 138.0 | 140.0 | 1.0144928 |
| Zinc sulfate (ZnSO4-7H2O) | 288.0 | 2.0E-4 | 6.9444445E-7 |
| Other Components | |||
| D-Glucose (Dextrose) | 180.0 | 2000.0 | 11.111111 |
| Glutathione (reduced) | 307.0 | 0.05 | 1.6286645E-4 |
| Methyl linoleate | 295.0 | 0.03 | 1.0169491E-4 |
| Sodium Pyruvate | 110.0 | 25.0 | 0.22727273 |