描述
这种IV型胶原酶利用溶组织梭菌制备。适用于细胞和组织解离。
IV型胶原酶推荐用于胰腺组织和胰岛的分离。
胶原酶是一种蛋白酶，能够特异性地结合Pro-X-Glyc-Pro序列中中性氨基酸 (x) 和甘氨酸之间的肽键。该序列高频率地存在于胶原中。胶原酶是唯一一种可以降解广泛存在于结缔组织中具有三股螺旋的天然胶原纤维的蛋白酶。
Life Technologies细胞培养产品系列的一员
Life Technologies提供了种类齐全的试剂，包括培养基、添加剂、盐溶液、缓冲液、化学品、解离试剂和转染试剂，可满足您的细胞培养需要。您可以信赖我们严格的质量控制和技术支持，确保使您满意。
仅供研究使用。不得用于人类或动物的治疗诊断。
Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.
Gibco® Collagenase Type IV is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and organ perfusions. Gibco® Collagenase Type IV activity is guaranteed to be greater than 160 units/mg. Compared to other collagenase preparations, Gibco® Collagenase Type IV has low tryptic activity and is well-suited for the digestion of islet cells from the pancreas.