纯化至多10 mg转染级质粒或柯斯质粒DNA
纯化得到相当于连续2次CsCl梯度离心得到的DNA纯度
高产量质粒DNA
经济的制备方法
使用LyseBlue，获得最佳的裂解效果和最大的DNA产量
QIAGEN Plasmid Kits应用重力流阴离子交换柱纯化转染级质粒DNA。该试剂盒可纯化获得至多10 mg的质粒DNA。离心获得澄清的裂解产物和异丙醇沉淀。
QIAGEN Plasmid Kits纯化质粒DNA适合多种应用，如：
转染克隆
PCR
体外转录
Features | Plasmid Giga Kit | Plasmid Mega Kit | Plasmid Maxi Kit | Plasmid Midi Kit | Plasmid Mini Kit |
|---|---|---|---|---|---|
| Applications | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. | Transfection, cloning, sequencing, capillary sequencing, etc. |
| Culture volume/starting material | 2.5–5 liters culture volume | 500 ml – 2.5 liters culture volume | 100–500 ml culture volume | 25–100 ml culture volume | 3–10 ml culture volume |
| Elution volume | Variable | Variable | Variable | Variable | Variable |
| Plasmid type | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA |
| Processing | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation) | Manual (centrifugation) |
| Sample per run | 1 sample per run | 1 sample per run | 1 sample per run | 1 sample per run | 1 sample per run |
| Technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology |
| Time per run | 320 min | 220 min | 160 min | 150 min | 80 min |
| Yield | <10 mg | <2.5 mg | <500 µg | up to 100 µg | <20 µg |
Procedure
With QIAGEN Plasmid Kits, bacterial lysates are cleared by centrifugation. The cleared lysate is then loaded onto the anion-exchange tip where plasmid DNA selectively binds under appropriate low-salt and pH conditions. RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart "QIAGEN Plasmid Kit procedures"). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation.
Applications
Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications, such as:
Transfection
Cloning
PCR
In vitro transcription
Specifications
Features | Specifications |
| Applications | Transfection, cloning, sequencing, capillary sequencing etc. |
| Culture volume/starting material | 2.5–5 liters culture volume |
| Plasmid type | High-copy, low-copy, cosmid DNA |
| Processing | Manual (centrifugation) |
| Samples per run (throughput) | 1 sample per run |
| Technology | Anion-exchange technology |
| Time per run or prep per run | 320 min |
| Yield | <10 mg |