快速纯化至多10 mg转染级质粒或柯斯质粒DNA
· 无需离心澄清裂解液
· 减少质粒纯化时间
· 高产率质粒DNA
· 使用LyseBlue,获得最佳的裂解效果和最大的DNA产量
QIAfilter Plasmid Kits基于阴离子交换技术纯化质粒DNA,细菌裂解液通过过滤来澄清。该试剂盒可纯化获得至多10 mg DNA。经纯化的DNA的纯度相当于两次CsCl梯度离心方法制备的纯度,适合转染级的应用。
Features | QIAfilter Plasmid Giga Kit | QIAfilter Plasmid Mega Kit | QIAfilter Plasmid Maxi Kit | QIAfilter Plasmid Midi Kit |
Applications | Transfection, cloning, sequencing, etc. | Transfection, cloning, sequencing, etc. | Transfection, cloning, sequencing, etc. | Transfection, cloning, sequencing, etc. |
Culture volume/starting material | 2.5 liters culture volume | 500 ml – 2.5 liters culture volume | 100–250 ml culture volume | 25–50 ml culture volume |
Plasmid type | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA | High-copy, low-copy, cosmid DNA |
Processing | Manual (filtration) | Manual (filtration) | Manual (filtration) | Manual (filtration) |
Sample per run | 1 sample per run | 1 sample per run | 1 sample per run | 1 sample per run |
Technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology | Anion-exchange technology |
Time per run | 280 min | 190 min | 120 min | 110 min |
Yield | <10 mg | <2.5 mg | <500 µg | <100 µg |